Amplification of pNK2 muted

The DH5α is transformed with this new product dialysed called pNK2 (2).
Transformation Pseudovibrio with pNK2 and sowed in selection medium kanamycin (50µg/mL) for to have a fresh transformed cells.

transposas Tn10

PCR Tn10 with primer biobrick 64 and 65 is accomplished. After migration a bands at 1200 bp. This product is purified and send to sequence.

sep 02