Team:USyd-Australia/Project/Design
From 2014.igem.org
Revision as of 17:10, 17 October 2014 by Andy.Bachler (Talk | contribs)
Project Design
Gene Cassettes are the carriers of genetic information in our design. They are small, modular chunks of DNA containing a gene flanked by AttC sites. Cassettes, unlike plasmids, are non-replicative and so do not require extra genes to modulate or initiate replication. To create a cassette simply requires the use of primers to create half AttC sites at either end and circularizing the product through Gibson Assembly or in other ways.