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From 2014.igem.org

Characterization of the Efficiency of the ATF1 Enzyme

The ATF1 enzyme converts isoamyl alcohol to isoamyl acetate, which gives off a strong banana odor. The conversion of isoamyl alcohol to isoamyl acetate can be monitored using gas chromatography and mass spectroscopy. Thus, the efficiency of the ATF1 enzyme can be characterized.

We have constructed two different biobricks that include the ATF1 gene. The first biobrick, K1423007, is a construct consisting of an arsenic inducible promoter, a ribosome binding site, the ATF1 gene, and a double terminator. The second biobrick, K1423008, consists of a high strength constitutive promoter, a ribosome binding site, the ATF1 gene, and the double terminator.

BBa_K1243007: Inducible Banana Odor Generator

BBa_K1243008: Constitutive Banana Odor Generator

Quantification of ATF1 through Gas Chromatography and Mass Spectroscopy

In order to efficiently quantify the performance of these two constructs, we designed and performed a number of experiments based around E.Coli with the constitutive promoter plasmid, and E.Coli with the arsenic inducible promoter. The full protocol is located in our protocol section, and can be located through this link. Some findings through these experiments revealed that: wq