Team:Groningen/Template/MODULE/Notebook/characterisation/week3

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15 - 21 September
 
Fusion of Nis A and Superfolded GFP with terminator
 
Single and double digestion of Nis A and Superfolded GFP to confirm the size of the plasmid
Ligation experiment with PSB1A3 as a backbone and B0015 as 2nd insert
Transformation of overnight ligated sample in E. coli
Size of the construct was determined with the help of colony PCR
Double digestion, purification of the construct
 
 
 
 
 

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