Team:Paris Bettencourt/Parts

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SMELL THE ROSES

SOMETHING FISHY


DON'T SWEAT IT

INTERLAB STUDY

Smell the roses

BBa_K1403003 - Limonene synthase

This part consists of a limonene synthase coding sequence downstream of a constitutive promoter.

Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent./p>

BBa_K1403006 - Jasmonic acid carboxyl methyltransferase

Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid

BBa_K1403009 - bmst1

BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.

BBa_K1403012 - alcohol acetyltransferase I

This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor

BBa_K1403017 - aldB

It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.

BBa_K1403019 - ilvBN

ilvBN gene exists in natural wild type E.Coli strain. They codes for acetolactate synthase process. Here, we isolated it from MG1655 strain through PCR to transform it into targeted strain for increased basal production of acetolactate, which serves as precursor for acetoin production.

Something fishy

BBa_K1403015 - Trimethylamine monooxygenase
Trimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form tryptophan.

Don't sweat it

Part 8

Interlab study

Part 9

Part 10

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com
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