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Figure 4:
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1. Top layer: Transparent polymethylpentene membrane permeable to gases, flexible and
2. Middle layer: Polyacrylamide hydrogel containing the chemically defined media and
3. Bottom layer: This layer acts as barrier for the Lactococcus lactis cells to be restrained hydrophobic. Lyophilized Lactococcus lactis cells. They are inactive since the gel is in dehydrated form. inside the bandage. Cellulose nitrate membrane of pore size 0.2 micrometers, permeable to protein, hydrophilic and flexible.
2. Middle layer: Polyacrylamide hydrogel containing the chemically defined media and
3. Bottom layer: This layer acts as barrier for the Lactococcus lactis cells to be restrained hydrophobic. Lyophilized Lactococcus lactis cells. They are inactive since the gel is in dehydrated form. inside the bandage. Cellulose nitrate membrane of pore size 0.2 micrometers, permeable to protein, hydrophilic and flexible.
To evaluate different bandage designs, we develop a multi-scale dynamic model of the bandage. The bandage is discretized into lattices where each lattice contains differential equations describing the growth of bacteria, production of nisin, production of Aiia, production of DspB and the detection of quorum molecules. Apart from the differential equations for the productions of the three IPM molecules we also consider the diffusion parameters. This makes the model more dynamic to study characteristics of our bandage. (Refer Figure 1 for all variables that are taken into account in the equations for each lattice).
Each state variable in each lattice is initialized according to the different bandage designs. Each lattice contains few bacteria which uses glucose as nutrient source and grows. Actively growing bacteria produce Nisin, Aiia and DspB only in response to the quorum molecules produced by both Staphylococcus aureus and Pseudomonas aeruginosa. In presence of quorum molecules in the lattice, the bacteria starts producing Nisin, Aiia and DspB. Nisin, Aiia and DspB produced in a lattice diffuses to nearby lattices until equilibrium is reached.
