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Team:Sumbawagen/Safety
From 2014.igem.org
Team:Sumbawagen/Team
From 2014.igem.org
Safety
- 1. Safety Rules in Laboratory
- 2. Safety Project
- 3. Safety Form
1.Safety rules in laboratory
The basic rules in our laboratory are:
a. Everyone who join the project has been trained and pass the evaluation before join to the laboratory experiment. The training covered several topics, such as basic information about IGEM and synthetic biology, technique of DNA isolation and handling, cutting and joining of DNA, plasmid transformation, handling of the synthetic biology waste and basic rules of laboratory safety
b. Everyone who working in the laboratory should following the basic standard of laboratory safety, such as wearing safety cloth (laboratory coat, rubber gloves, and surgery mask) while working in the experiment according to the risk level type.
c. In order to minimize the contamination, we done the experiment in laminar air flow (clean bench), following the standard procedure.
d.Laboratory activity were done by the students under supervision of instructors.
2. Safety Project
We are working with 2 type of E. coli strain, they are E. coli BL21 (DE3) and E. coli DH5α. Both of strain are classified in biosafety level 1 and categorized as risk group number 1 (low risk). It does not cause disease in healthy adult humans and also does not have any additional safety precautions. We handle the organism safely by keep it into the safe place in our laboratory. We work in the restricted laboratory where not all people have access to enter the laboratory.
The basic rules in our laboratory are:
Biological wastes were produced as the by product of laboratory activity. In order to dispose the biological wastes (for example used pipette tip), according the standard of laboratory safety, we collect them together into certain box with label and decontaminated the autoclave before disposal or destroyed; the liquid waste such as cell culture and agar plate treated by decontamination with autoclave according to the laboratory standard operational procedures and dispose the material to the sanitary sewer system.
We use lab coat, rubber gloves and surgery mask in order to minimize contamination and hazard of the experiment wether in the laboratory or in the society (when the workshop activity taken place)
3. About our safety
- Link to "our lab form" https://igem.org/safety/about_our_lab?team_id=1508:
- Link to “safety form” https://igem.org/safety/safety_form?team_id=1508
- Link to “check-in” https://igem.org/safety/check_in?team_id=1508
The basic rules in our laboratory are:
a. Everyone who join the project has been trained and pass the evaluation before join to the laboratory experiment. The training covered several topics, such as basic information about IGEM and synthetic biology, technique of DNA isolation and handling, cutting and joining of DNA, plasmid transformation, handling of the synthetic biology waste and basic rules of laboratory safety
b. Everyone who working in the laboratory should following the basic standard of laboratory safety, such as wearing safety cloth (laboratory coat, rubber gloves, and surgery mask) while working in the experiment according to the risk level type.
c. In order to minimize the contamination, we done the experiment in laminar air flow (clean bench), following the standard procedure.
d.Laboratory activity were done by the students under supervision of instructors.
2. Safety Project
We are working with 2 type of E. coli strain, they are E. coli BL21 (DE3) and E. coli DH5α. Both of strain are classified in biosafety level 1 and categorized as risk group number 1 (low risk). It does not cause disease in healthy adult humans and also does not have any additional safety precautions. We handle the organism safely by keep it into the safe place in our laboratory. We work in the restricted laboratory where not all people have access to enter the laboratory.The basic rules in our laboratory are:
Biological wastes were produced as the by product of laboratory activity. In order to dispose the biological wastes (for example used pipette tip), according the standard of laboratory safety, we collect them together into certain box with label and decontaminated the autoclave before disposal or destroyed; the liquid waste such as cell culture and agar plate treated by decontamination with autoclave according to the laboratory standard operational procedures and dispose the material to the sanitary sewer system.
We use lab coat, rubber gloves and surgery mask in order to minimize contamination and hazard of the experiment wether in the laboratory or in the society (when the workshop activity taken place)
3. About our safety
- Link to "our lab form" https://igem.org/safety/about_our_lab?team_id=1508:
- Link to “safety form” https://igem.org/safety/safety_form?team_id=1508
- Link to “check-in” https://igem.org/safety/check_in?team_id=1508
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