Team:DTU-Denmark/Primers
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Revision as of 12:40, 17 October 2014 by KristianJensen (Talk | contribs)
Below is a list of primers used in the experimental work
Primer name | 5'-sequence-3' | Description |
VF2 | TGCCACCTGACGTCTAAGAA | Primer for sequencing insert in standard backbone |
VR | ATTACCGCCTTTGAGTGAGC | Reverse primer for sequencing insert in standard backbone |
P3 | ATTCCGCUAAGGATGATTTCTGGAATTCGCGG | Primer attaching on backbone upstream BioBrick prefix |
P4 | ACCTTGCCCUTTTTTGCCGGACTGCAGCGGC | Primer attaching on backbone downstream BioBrick suffix |
P5 | TGGCGCCCGAACAGGGACTTGA | Reverse primer attaching upstream terminator for in vitro template amplification |
P6 | TAATACGACTCACTATAGGGAGAGCCCGGATAGCTCAGTCGGT | Primer with T7 promoter tail for in vitro transcription template aplification |
P7 | TTGCCGGACTGCAGCGGCCGCTACTAGTATGGCGCCCGAACAGGGACTT | Reverse primer attaching upstream of the terminator with a primer tail including BioBrick suffix |