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Team:Paris Saclay/Notebook/June/30
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Contents |
Monday 30th June
Lab work
1 - Culture or the supercompetents:
Mathias
- MG1655
- MG1655Z1
1mL of an overnight culture in 100mL of LB.
2 - Réhydration of selected biobricks:
Juliette
- BBQ_I9026
- BBQ_K592025
- BBQ_K786002
- BBQ_K786003
- BBQ_K778002
- BBQ_K592011 {2 times}
- BBQ_K592009
- BBQ_K1033910 {idem}
- BBQ_J45014
- BBQ_K517003
3 - Transformations :
Maher & Mathias
We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.
4 - Preparations :
- Aliquots of antibiotics :
Marie & Mathieu
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)
- Petri dishes
Romain
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
People there:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
