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Team:Carnegie Mellon/Superoxide
From 2014.igem.org
Superoxide Generator Improvement
iGEM Registry of Standard Biological Parts, Part:BBa K1184000
In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods(Source: Carnegie Mellon iGEM 2013)
Methods Used
- Optimization of codons in KillerRed by eliminating rare codon usage.
- Optimization of codons in Supernova, the monomeric version of KillerRed (Takemoto et al. 2013), by eliminating rare codon usage.
- Photobleaching analysis of KillerRed, Codon Optimized KillerRed, and Codon Optimized Supernova.
Kiwamu Takemoto, Tomoki Matsuda, Naoki Sakai, Donald Fu, Masanori Noda, Susumu Uchiyama, Ippei Kotera, Yoshiyuki Arai, Masataka Horiuchi, Kiichi Fukui, Tokiyoshi Ayabe, Fuyuhiko Inagaki, Hiroshi Suzuki & Takeharu Nagai. 2013. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation, Scientific Reports; 3, 1-7
