Nitrosomonas europaea

• obtained starter culture from Freddy Chen from George Wells's Lab in the Environmental Engineering department
• it is grown in the dark, at 30C, in a special buffer called NE buffer (has specific concentrations of ions)
• instead of measuring its OD, (researchers have not been able to culture it past 0.1), we monitored growth by pH
• the starting pH should be around 7.5, after about 21 days, the pH will drop to about 6, at which point the culture will need to be split into new flasks

Streptomyces fluorescens