Team:UIUC Illinois/Notebook

Title

ASDF

Monday 9^th June

Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2O
Autoclaved 1.5 mL Eppendorf tubes as well as LB
Poured plates of LB and chloramphenicol (CAM)
Streaked 2 plates with E. coli DguaB (pDCAF3)
Incubated in 37 degrees celsius
Aliquoted kanamycin (KAN) in four 1mL tubes

Title

ASDF

Tuesday 10^th June

Autoclaved at 1:30pm

• 0.8 grams of YNB + 156 mL H2O
• 28 grams of salts + 490 mL H2O

Used sterile filter for 1M MgSO4 and 1M CaCl2
Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli
Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture

• Labeled this "M9 media"

Inoculated frozen stock of E. coli guaB pDCAF3 in 5 mL of M9 media
Placed sample in 30 degree celsius shaker for culturing