Team:UIUC Illinois/Notebook
From 2014.igem.org
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DAY NOTES
- NOTES
Title
ASDFMonday 9th June
Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2OAutoclaved 1.5 mL Eppendorf tubes as well as LB
Poured plates of LB and chloramphenicol (CAM)
Streaked 2 plates with E. coli DguaB (pDCAF3)
Incubated in 37 degrees celsius
Aliquoted kanamycin (KAN) in four 1mL tubes
Title
ASDFTuesday 10th June
Autoclaved at 1:30pm- 0.8 grams of YNB + 156 mL H2O
- 28 grams of salts + 490 mL H2O
Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli
Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture
- Labeled this "M9 media"
Placed sample in 30 degree celsius shaker for culturing