Team:Groningen/Template/MODULE/Notebook/toolbox/week11

SEQUENCING RESULTS

A PCR was done to generate fragments for assembling genes that had illegal restriction sites. Every fragment was succesfully amplified. The fragments were purified using the GeneJET PCR clean up kit. were not fully clean, so only the fragments for the combined BioBrick of NisF, NisE and NisG was made using Gibson assembly. The transformants that contained pSB1C3 with NisFEG were tested using colony PCR. No positive transformants were found.