Team:Cambridge-JIC/Guide/Constructs/Reporters

From 2014.igem.org

The following fluorescent proteins have been used with consistent results in MP: [http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP.

A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: [http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI].

These can be added instead of the stop codon for the reporters

These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include:

LTI

Confocal Microscopy image of eGFP n7. Excitation:488, Detection range: 520-540.

Fluorescent transformants are visible first from 3-5 days after transformation.

GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper]