Team:Evry/Interlab Study/09-14-2014

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Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED

PSB1C3+E0240+J23106:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C.
PSB1C3+E0240+J23114, PSB1C3+E0240+J23115 and PSB1C3+E0240+J23116:
  • PCR colony of 5 colonies for each construction following protocol table 1 and 2.
    IMAGE
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    GEL 1: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23114 construction.

    IMAGE
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    GEL 2: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.
Sep 14

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