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From 2014.igem.org

Results / Heavy metals / Arabidopsis thaliana T4MBP

Labwork

Transformation of A. thaliana with our T4MBP:

1. Exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using resctriction site based cloning.
2. We integrated our T4MBP in our modified pORE_E3_2x35S.
3. Application of our floral dip method.
4. After 6 weeks we harvested transgenic seeds from A. thaliana and plated these seeds on selection MSO-media.
5. At last we potted transformed plants.

Results

The exchange of the promoter was checked by sequencing. We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

Fig. 2: Pictures showing the vector pORE_E3 with 2x35S promoter and the way of exchanging the enTCUP2 promoter with the 2x35S promoter. Each step that have been done to exchange these promoters is shwon in this histroy .