<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014

Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL

Background information

Systems considered

Modeling leakiness

Results

Characterization of two-level crosstalk on the promoter

Background information

System considered

Modeling crosstalk

First-order crosstalk

First Level crosstalk: LuxR binds to different HSL and activates the promoter

Second Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter

Second order crosstalk: Combination of both cross-talk levels

Other regulatory proteins, like LasR, bind to different HSL and activates the promoter

Results

Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration

Background information

Systems considered

Modeling promoter's sensitivity

Results