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English Abstract: LactoAid - A smart bandage for burnwounds
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
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Chinese Abstract: LactoAid-给灼伤一个聪明的敷料
金黄色葡萄球菌和绿脓杆菌经常导致灼伤伤口感染,影响治疗。本研究研制了一种敷料,既
可以预防此类感染,又能减少抗生素的使用,因此降低抗生素抗药性的发生。此敷料带有一
种水凝胶,里面含有转基因乳酸乳球菌和它所需要的营养成分。转基因的乳酸乳球菌能够探
测到灼伤伤口里两种病原体的群体感应分子,并发出乳酸链球菌肽以及另外两种抗感染分子
(Infection Preventing Molecules, IPMs)。这两种抗感染分子分别是分散蛋白(Dispersin B)和群
体感应淬灭酶酰基高丝氨酸内酯酶。水凝胶夹在两层中间,上层使敷料透气,下层控制敷料
里的细菌。通过把邻近的水包穿破,使水凝胶产生水合反应,因而使细菌开始生长,这样就
可以激活此敷料了。
French Abstract: LactoAid - A smart bandage for burnwounds
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
English Abstract: LactoAid - A smart bandage for burnwounds
English Abstract: LactoAid - A smart bandage for burnwounds
Dutch Abstract: LactoAid - Een slim verband voor brandwonden
Infecties door Staphylococcus aureus en Pseudomonas aeruginosa zorgen vaak voor complicaties bij de behandeling van brandwonden. We hebben een nieuw soort verband ontwikkeld dat deze infecties tegengaat en het gebruik van antibiotica terugdringt waardoor het risico op de ontwikkeling van antibiotica resistentie verminderd wordt. Het verband bestaat uit een hydrogel die genetisch gemodificeerde Lactococcus lactis bevat met nutriënten. Deze gemodificeerde stam van L. lactis detecteert 'quorum sensing' moleculen van de twee pathogenen in de wond en produceert vervolgens zowel het antimicrobiële nisine als enkele andere Infection-Preventing-Molecules (IPMs). Deze IPMs zijn het anti-biofilm eiwit Dispersin B en het quorum quenching eiwit AHLase. The gel zit tussen twee lagen, een bovenlaag die de diffusie van gassen accomodeert en een onderlaag om de bacteriën in het verband vast te houden. De groei van bacteriën begint als de gel bevochtigd wordt door naastliggende waterzakjes te breken, zodat het verband geactiveerd wordt.
Infecties door Staphylococcus aureus en Pseudomonas aeruginosa zorgen vaak voor complicaties bij de behandeling van brandwonden. We hebben een nieuw soort verband ontwikkeld dat deze infecties tegengaat en het gebruik van antibiotica terugdringt waardoor het risico op de ontwikkeling van antibiotica resistentie verminderd wordt. Het verband bestaat uit een hydrogel die genetisch gemodificeerde Lactococcus lactis bevat met nutriënten. Deze gemodificeerde stam van L. lactis detecteert 'quorum sensing' moleculen van de twee pathogenen in de wond en produceert vervolgens zowel het antimicrobiële nisine als enkele andere Infection-Preventing-Molecules (IPMs). Deze IPMs zijn het anti-biofilm eiwit Dispersin B en het quorum quenching eiwit AHLase. The gel zit tussen twee lagen, een bovenlaag die de diffusie van gassen accomodeert en een onderlaag om de bacteriën in het verband vast te houden. De groei van bacteriën begint als de gel bevochtigd wordt door naastliggende waterzakjes te breken, zodat het verband geactiveerd wordt.
Finnish Abstract: LactoAid/LaktoApu Älyside palovammoille
Staphylococcus aureus ja Pseudomonas aeruginosa bakteerien infektiot aiheuttavat usein ongelmia palovammojen hoidossa. Me kehitimme uudenlaisen siteen, joka ehkäisee näitä infektioita ja vähentää antibioottien käyttöä, ja siten vähentää antibioottiresistenssin kehittymisen riskiä. Siteessä on ravinteita sisältävää hydrogeeliä, jossa on geneettisesti paranneltua Lactococcus lactis bakteeria. Tämä paranneltu kanta tunnistaa näiden kahden patogeenin erittämiä viestimolekyylejä haavassa, minkä seurauksena se tuottaa antimikrobista nisiiniä sekä infektiota estäviä molekyylejä, biofilminestäjäproteiini Dispersiini B:tä sekä patogeenien viestintää tukahduttavaa AHLaasia. Geeli sijaitsee kahden kerroksen välissä; päällimmäinen kerros hengittää ja alempi kerros estää bakteerien pääsyn geeliltä iholle. Kun siteessä olevat vesitaskut puhkaisee, geeli kostuu ja käynnistää bakteerien kasvun, jolloin älyside aktivoituu.
Staphylococcus aureus ja Pseudomonas aeruginosa bakteerien infektiot aiheuttavat usein ongelmia palovammojen hoidossa. Me kehitimme uudenlaisen siteen, joka ehkäisee näitä infektioita ja vähentää antibioottien käyttöä, ja siten vähentää antibioottiresistenssin kehittymisen riskiä. Siteessä on ravinteita sisältävää hydrogeeliä, jossa on geneettisesti paranneltua Lactococcus lactis bakteeria. Tämä paranneltu kanta tunnistaa näiden kahden patogeenin erittämiä viestimolekyylejä haavassa, minkä seurauksena se tuottaa antimikrobista nisiiniä sekä infektiota estäviä molekyylejä, biofilminestäjäproteiini Dispersiini B:tä sekä patogeenien viestintää tukahduttavaa AHLaasia. Geeli sijaitsee kahden kerroksen välissä; päällimmäinen kerros hengittää ja alempi kerros estää bakteerien pääsyn geeliltä iholle. Kun siteessä olevat vesitaskut puhkaisee, geeli kostuu ja käynnistää bakteerien kasvun, jolloin älyside aktivoituu.
Hindi Abstract: LactoAid - A smart bandage for burnwounds
स्टेफाईलोकॉकस औरीयस और सूडोमोनस एरोगिनोसा की वजह से संक्रमण अक्सर जला घाव उपचार के लिए समस्या पैदा कऱता है। हमने एक नए प्रकार की पट्टी का विकास किया है, जो संक्रमणों से बचाता है और एंटीबायोटिक दवाओं के उपयोग को कम कर देता है और इस तरह एंटीबायोटिक प्रतिरोध विकसित होने का खतरा कम कर देता है
स्टेफाईलोकॉकस औरीयस और सूडोमोनस एरोगिनोसा की वजह से संक्रमण अक्सर जला घाव उपचार के लिए समस्या पैदा कऱता है। हमने एक नए प्रकार की पट्टी का विकास किया है, जो संक्रमणों से बचाता है और एंटीबायोटिक दवाओं के उपयोग को कम कर देता है और इस तरह एंटीबायोटिक प्रतिरोध विकसित होने का खतरा कम कर देता है
English Abstract: LactoAid - A smart bandage for burnwounds
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
English Abstract: LactoAid - A smart bandage for burnwounds
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
