Team:NEAU-Harbin/protocols.html

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1.PCR amplification

① PCR amplification of amilCP (BBa_K592009)
Primers:
Sense: GGGCCCATGAGTGTGATCGCTAAACAAAT
Antisense: GGTACCAAGCTTAGGCGACCACAGGTTTG
Reaction system:

PCR condition:

② PCR amplification of cjBlue (BBa_K592011)
Primers:
Sense: GAATTCGGTAGCGGCTCCGGAAGCGGTTCCGGCAGCatggcttccaaaataagcg
Antisense: GGTACCAAGCTTAGGCGACCACAGGTTTG
Reaction system:

PCR condition

③ PCR amplification of GlaA3
Primers:
Sense: AGATCT ACAATCAATCCATTTCGCTATAG
Antisense: TCTAGA CATAAGGCGGGTTCACATC
Reaction system:

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