Team:UCLA/Notebook/Protocols

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iGEM UCLA




Protocols
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
Equipment for the collection of recombinant silk Equipment Protocol
List of Reagents Reagent Protocol
PCR for MASP1 and MASP2 PCR Protocol
Silk Fibroin Extraction Silk Fibroin Extraction Protocol
Silk Films Silk Films Protocol
Electrospun Fibers Electrospun Fiber Protocol

Reference Materials Fabrication Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web. Production of MASP1/2 Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.