Team:Tuebingen/Notebook/Protocols/restriction

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Preparative restriction digests

Reagents

min. 2 µg Plasmid DNA
2 µL of each restriction enzyme
10 µL 10x NE buffer 2
1 µL 100x BSA
to 100 µL H2O
0.5 µL Pfu Polymerase (1 U/µL)
to 50 µL H20

Procedure

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