"
Team:ITB Indonesia/nb-wetlab
From 2014.igem.org
June
Week 1
First week of june we got look arround the laboratory space that we would using and make a list of preparation that we need for wetlab team. We prepare for LB medium, antibiotic optimation and make competent cell using CCMB 80 buffer. We also sterilize all of the equipment needed. We have already choose our idea to engineer bacteria that could degrade plastic. Before choosing that idea we had four main ideas:
1. Microalgae biofuel
2. Synthetic bacteria that could degrade plastic
3. Urine biosensor
4. Sianide biosensor
After competent cells are ready and antibiotic optimation done we have plan to try rehydrated some parts and transform it to our E. coli DH5α competent cell. We also make a design of our construct whic is become the final design after long discussion before so that we will ready to order some synthetic part and order to iGEM Hq for some part that not provided in the 2014 parts.
Week 2
This is our first time to see the iGEM distribution kit plate so exciting. We will make a trial to transform iGEM 2013 distribution kit using our competent cell. That is not work good on first, second and third trial. Then we concluded that we must try the iGEM distribution kit plate 2014. We also try to test our competent cell efficiency and we dont face any problem with our competent cell.
Week 3
Finnaly, we rehydrated parts from iGEM kit plate 2014 which have many twins to make sure our methode is optimal to transform iGEM parts. We have already planed to make four system for our bacteria degrading plastic so that we devided our team into four group that will concerning to each system. We try to transform BBa_K592025, BBa_B0015, BBa_B0034, BBa_I0040. After the transformation still we dont have any colonies.
Week 4
We have target that we must be abble to transform parts from iGEM distribtion kit. Finnaly we can see our red colony from RFP parts. So, we make liquid culture and wait until 16 hours, the medium turn into red. We try to isolation the plasmid using Alkaline Lysis method. Confirmation of the plasmid isolation using electrophoresis, restriction and PCR. The result was positive.
June
Week 1
First week of june we got look arround the laboratory space that we would using and make a list of preparation that we need for wetlab team. We prepare for LB medium, antibiotic optimation and make competent cell using CCMB 80 buffer. We also sterilize all of the equipment needed. We have already choose our idea to engineer bacteria that could degrade plastic. Before choosing that idea we had four main ideas:
1. Microalgae biofuel
2. Synthetic bacteria that could degrade plastic
3. Urine biosensor
4. Sianide biosensor
After competent cells are ready and antibiotic optimation done we have plan to try rehydrated some parts and transform it to our E. coli DH5α competent cell. We also make a design of our construct whic is become the final design after long discussion before so that we will ready to order some synthetic part and order to iGEM Hq for some part that not provided in the 2014 parts.
Week 2
This is our first time to see the iGEM distribution kit plate so exciting. We will make a trial to transform iGEM 2013 distribution kit using our competent cell. That is not work good on first, second and third trial. Then we concluded that we must try the iGEM distribution kit plate 2014. We also try to test our competent cell efficiency and we dont face any problem with our competent cell.
Week 3
Finnaly, we rehydrated parts from iGEM kit plate 2014 which have many twins to make sure our methode is optimal to transform iGEM parts. We have already planed to make four system for our bacteria degrading plastic so that we devided our team into four group that will concerning to each system. We try to transform BBa_K592025, BBa_B0015, BBa_B0034, BBa_I0040. After the transformation still we dont have any colonies.
Week 4
We have target that we must be abble to transform parts from iGEM distribtion kit. Finnaly we can see our red colony from RFP parts. So, we make liquid culture and wait until 16 hours, the medium turn into red. We try to isolation the plasmid using Alkaline Lysis method. Confirmation of the plasmid isolation using electrophoresis, restriction and PCR. The result was positive.
June
Week 1
First week of june we got look arround the laboratory space that we would using and make a list of preparation that we need for wetlab team. We prepare for LB medium, antibiotic optimation and make competent cell using CCMB 80 buffer. We also sterilize all of the equipment needed. We have already choose our idea to engineer bacteria that could degrade plastic. Before choosing that idea we had four main ideas:
1. Microalgae biofuel
2. Synthetic bacteria that could degrade plastic
3. Urine biosensor
4. Sianide biosensor
After competent cells are ready and antibiotic optimation done we have plan to try rehydrated some parts and transform it to our E. coli DH5α competent cell. We also make a design of our construct whic is become the final design after long discussion before so that we will ready to order some synthetic part and order to iGEM Hq for some part that not provided in the 2014 parts.
Week 2
This is our first time to see the iGEM distribution kit plate so exciting. We will make a trial to transform iGEM 2013 distribution kit using our competent cell. That is not work good on first, second and third trial. Then we concluded that we must try the iGEM distribution kit plate 2014. We also try to test our competent cell efficiency and we dont face any problem with our competent cell.
Week 3
Finnaly, we rehydrated parts from iGEM kit plate 2014 which have many twins to make sure our methode is optimal to transform iGEM parts. We have already planed to make four system for our bacteria degrading plastic so that we devided our team into four group that will concerning to each system. We try to transform BBa_K592025, BBa_B0015, BBa_B0034, BBa_I0040. After the transformation still we dont have any colonies.
Week 4
We have target that we must be abble to transform parts from iGEM distribtion kit. Finnaly we can see our red colony from RFP parts. So, we make liquid culture and wait until 16 hours, the medium turn into red. We try to isolation the plasmid using Alkaline Lysis method. Confirmation of the plasmid isolation using electrophoresis, restriction and PCR. The result was positive.
