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University of Reading
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Genetic modification in
Synechocystis
is done by modifying the chromosome,
rather than inserting plasmids. Synechocystis is na
turally transformable, and will
undergo homologous recombination between its chromo
some and a plasmid
containing a homologous region. This means that pla
smids for insertion or
deletion need to have regions of ~500 to ~1000bp ei
ther side of the inserted
sequence, making modifications time consuming or co
stly depending on your
method of plasmid construction. We will therefore s
ubmit all our BioBricks for
insertions and deletions to the registry. All of th
ese will contain Kanamycin
resistance for selecting transformants.
The mechanism for each of the BioBricks is the same
. They will undergo
recombination with the region that they share homol
ogy with. Knockouts will
undergo recombination with the specified gene, repl
acing it will kanamycin
resistance. Insertions will undergo recombination w
ith a region of the
chromosome that is not important for the metabolic
conditions we are using, and
will insert the gene of interest and a kanamycin re
sistance gene.
We are creating 7 BioBricks that will be submitted
to registry, consisting of 4
deletions and 3 insertions, in addition to improvin
g characterization of an
existing BioBrick. Background information on the ai
m of these parts can be
found in our “Project” section.
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Parts Table |
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Any parts your team has created will appear in this table below: |
<groupparts>iGEM013 Reading</groupparts>
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