"

From 2014.igem.org

University of Reading
Home Team Project Fuel Cell Parts Human Practices Lab book Protocols Attributions

Genetic modification in Synechocystis is done by modifying the chromosome, rather than inserting plasmids. Synechocystis is na turally transformable, and will undergo homologous recombination between its chromo some and a plasmid containing a homologous region. This means that pla smids for insertion or deletion need to have regions of ~500 to ~1000bp ei ther side of the inserted sequence, making modifications time consuming or co stly depending on your method of plasmid construction. We will therefore s ubmit all our BioBricks for insertions and deletions to the registry. All of th ese will contain Kanamycin resistance for selecting transformants. The mechanism for each of the BioBricks is the same . They will undergo recombination with the region that they share homol ogy with. Knockouts will undergo recombination with the specified gene, repl acing it will kanamycin resistance. Insertions will undergo recombination w ith a region of the chromosome that is not important for the metabolic conditions we are using, and will insert the gene of interest and a kanamycin re sistance gene. We are creating 7 BioBricks that will be submitted to registry, consisting of 4 deletions and 3 insertions, in addition to improvin g characterization of an existing BioBrick. Background information on the ai m of these parts can be found in our “Project” section.

Parts Table

Any parts your team has created will appear in this table below:

<groupparts>iGEM013 Reading</groupparts>

Facebook

Twitter

rusynbioigem@gmail.com