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Team:NCTU Formosa/Notebook/test5
From 2014.igem.org
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8/11
Transformation of NCTU1 (G1)
Transformation of PBAN (G2)
8/12
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr
Mini-prep of [Pcons]Ar
Digestion of [Pcons]Ar (ES) (G2)
8/13
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP)
Ligation of[Pcons]+[RBS+BFP+J61048] (G2)
8/14
Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
Purification from electrophoresis of[PBAN]No.4&5&6(G1)
Transformation of the ligation product made yesterday
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Purification from electrophoresis of [PBAN]No.4&5&6
Transformation of [B0034]Ar (G2)
8/16
Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)
PCR of [B0034]
Electrophoresis of[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034
Mini-prep of [B0034]Ar E.coli
Ligation of [Pcons]+[B0034] +PSB1K3
Digestion of [BFP](ES)
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])
8/17
Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)
