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From 2014.igem.org

Protocols / SDS-PAGE

Material:
12 % Running gel	2.1 ml H2O, 2.5 ml acrylamide 4K 29:1 mix (40 %), 1.6 ml 1.5 M Tris-HCl pH 8.8, 62.5 μl 10 % SDS, 62.5 μl 10 % APS, 2.5 μl TEMED
12 % Stacking gel	2.7 ml H2O, 680 μl acrylamide 4K 29:1 mix (40 %), 540 μl 1 M Tris-HCl pH 6.8, 40 μl 10 % SDS, 30 μl 10 % APS, 8 μl TEMED
Electrophoresis system
Running buffer	250 mM Tris-HCl pH 8.3, 1.92 mM glycine, 0,1 % (v/v) SDS