Team:Brasil-SP

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Project Description

     Our project consists of a biological molecular device for detection of early stage chronic kidney disease using Cystatin C as biomarker and Bacillus subtilis as chassis. The genetic circuit being assembled is based on the outstanding project of iGEM 2010 Imperial College of London team (special thanks to the ex-iGEMer Christopher Hirst, who helped us a lot sending some important BioBricks). Part of our mission is also to improve characterization of the BioBricks developed in 2010 and to validate the molecular design as Cystatin C detection system. This flexibility of detection is based on a protease cleavage of a membrane protein which triggers the genetic circuit. Since any cleavage site could be designed, virtually any protease could be used as a signal for the detection. In our case, the disease biomarker will inhibit the action of Cathepsin S protease and the detection will be made indirectly and negatively - i.e. by the Cathepsin lack of protease activity and absense of the system output. We are on the way to assemble all the parts and properly characterize each part of our construction on time for the Jamboree.
     To address a real world situation, we are working on the same principle and aesthetics of the well known devices for biodetection like pregnancy or HIV tests: easy-to-use microfluidic devices. The plan is to design a microchip able to store spores of the developed strains of B. subtilis and safely expose blood samples to our biodetection system, successfully containing the biomaterial and enabling a proper discard of the chip. A priori, the device output monitoring would require a fluorescence detector tool, but we also propose a naked eye output observation as a concept for future prospects.
      Since we are working on a solution for a problem directly related to ordinary people, having a public feedback about synthetic biology is very important to analyze the social impact of our work and it help us to evaluate the biosafety and bioethical issues beyond a simple risk analysis - a sociological characterization of the values of our project. Thus, as a policy and practices approach, we will try to report Brazil's public opinion on these issues using a questionnaire to evaluate our actual scenario and, in a certain way, our own project.

Wiki Pre Structure (Under Construction!)

This is the initial wiki pre structure that might be changed over its development. Everything here is merely provisional!

Home
The Issue
The Project
Modeling (Question: how many pages we'll really need here!? Modelling guys must have the answer.)
Results
Outreach (The "Human Practices" part)
Notebook
Team
- Members ("Team" + "Team Official Profile" (linking it) + "Attributions")
- Collaborations (Maybe the page "Working Together" is enought for this subject, right!?)
- Sponsors & Support

Requirements

Please be sure to keep these links, your audience will want to find your:

There are a few wiki requirements teams must follow:

All pages, images and files must be hosted on the 2014.igem.org server.
All pages must be created under the team’s name space.
As part of your documentation, keep the links from the menu to the left.
Do not use flash in wiki code.
The iGEM logo should be placed on the upper part of every page and should link to 2014.igem.org.

Visit the Wiki How To page for a complete list of requirements, tips and other useful information.

Tips

We are currently working on providing teams with some easy to use design templates.
In the meantime you can also view other team wikis for inspiration! Here are some very good examples

For a full wiki list, you can visit iGEM 2013 web sites and iGEM 2012 web sites lists.

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

State your accomplishments! Tell people what you have achieved from the start.
Be clear about what you are doing and what you plan to do.
You have a global audience! Consider the different backgrounds that your users come from.
Make sure information is easy to find; nothing should be more than 3 clicks away.
Avoid using very small fonts and low contrast colors; information should be easy to read.
Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2013 calendar
Have lots of fun!