"
Team:TU Eindhoven/Modeling/Cell Encapsulation Modeling
From 2014.igem.org
Cell Encapsulation Modeling
The modeling group focused on the microfluidics. With microfluidics the cells are separated into a single droplet. This process however is not very accurate. Sometimes the droplets do not contain the desired number of cells. This can be modelled using a Poisson distribution. In this model you can define an average number of cells per droplet, lambda. Lambda was varied to choose a suitable average number of cells per droplet for our microfluidic device.
Figure 1. Poisson distribution for multiple lambdas.
This allows for the determination of the ratios between one cell/zero cells and one cell/ two or more cells. These both have to be as high as possible for a specific lambda.
Figure 2. Ratio between one cell/ zero cells or more cells
Based on these results a lambda of 0.5 was chosen for the microfluidic device. The Poisson distribution for different number of cells becomes then as follows.
Figure 3.
