Team:Evry/Notebook/Transformation/08-27-2014

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Picture

Amplification of pRhokHI-2 in E.Coli Bl21
The transformation of E.Coli with pRhokHi-2 still didn't works. We still have to amplify it so we re-tried to the tranformation (1µL of plasmid for 40µL of cells, 1800V).
This time, after a liquid culture of only one hours cells were plated on LB 1X + Kan (1:1000), LB 1X + Cam (1:1000), and LB only and let in incubator overnight.

Aug 27

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