SURF - Synthetic Units for Redirecting Functionalities

Life’s success story on earth is largely based on the rewiring of a rather limited set of naturally occurring protein modules. We systematically analyzed the structural evolution of certain protein families by a computational approach, and deduced design principles for the creation of artificial scaffolds and catalysts with individualized properties. By this approach, we enable exchangeable and extendable protein libraries that carry synergistic functionalities. Our conceptual approach is challenged by the creation of chimeric proteins and their functional assessment as relevant biotechnological and biomedical applications for the sake of humankind.

To fulfill our mission we chose the following projects:

SilverSURF: Flagellin diversity as hub for synthetic scaffolds

Environmental pollution with harmful quantities of heavy metal ions has become an urgent problem in these times. SilverSURF makes use of the flagellar filament that represents one of the largest scaffolds in nature, and combines it with the unique potential of high-affinity metal-binding domains. Specifically, we rationally designed synthetic flagellar filaments able to bind huge quantities of the metal ion silver and copper. (Link zu unserem Brick + Cup1 von UtahState).

CancerSURF: DARPin based scaffolds for biomedical applications

CancerSURF

RiboSURF: Empowering the synthetic scope of the ribosome

Ribosomes synthesize proteins from amino acids using mRNA as blueprint and tRNAs delivering amino acids. However, the natural ability of ribosomes is limited to 20 L-amino acids. Non-ribosomal peptide synthetases (NRPSs) synthesize only small peptides, however, from a large number of natural and non-proteinogenic amino acids. RiboSURF aims at combining the huge repertoire of NRPSs with ribosomal protein synthesis to extend the synthetic scope of the ribosome. Specifically; we designed and probed the potential of synthetic catalysts that allow the aminoacetylation of tRNAs with non-proteinogenic amino acids.Ribosomes synthesize proteins from amino acids using mRNA as blueprint and tRNAs delivering amino acids. However, the natural ability of ribosomes is limited to 20 L-amino acids. Non-ribosomal peptide synthetases (NRPSs) synthesize only small peptides, however, from a large number of natural and non-proteinogenic amino acids. RiboSURF aims at combining the huge repertoire of NRPSs with ribosomal protein synthesis to extend the synthetic scope of the ribosome. Specifically; we designed and probed the potential of synthetic catalysts that allow the aminoacetylation of tRNAs with non-proteinogenic amino acids.