Team:SDU-Denmark/Notebook

Lab Journal

Week 25 (16/6 - 22/6)

Monday 16/6

The lab crash course began. We talked a lot about the project goal and the way of getting there. The team is now on the same page and ready for a great summer of exciting and interesting work to begin. In the lab we learned where everything was located and learned how to run a PCR.

Tuesday 17/6

In the lab: The PCR from yeasterday didn't work, so they were repeated. This time 50 µL PCR reaction were prepared. Gel purification was performed on the PCR products and the final concentration was measured by nanodrop. We digested the products by using restriction enzymes XbaI og SpeI. We detected that the plasmid was only linearized, meaning that only one restriction enzyme/ restriction site was functional. We worked on the basics in the iGEM concept. We discussed the definitions of biobrick, basis part, composite part and deviced. We learned the "Standard Assembly Method", it's condition, uses and limitations. We defined the conditions for our own device and started searching for the necessary parts in the iGEM registry

Wednesday 18/6

The XbaI enzyme didn't work to cut plasmid 161 tuesday and we wanted to find out what was wrong. We had different theories as to what went wrong tuesday:

• Maybe the XbaI didn't have enough time to cut the plasmid. We only gave it 5 minutes.
• Maybe the restriction site on plasmid 161 didn't work.

1. We wanted to do the same as on tuesday, but allow the enzyme to work for 15 minutes instead of 5 minutes, in case this was our mistake.
2. We wanted to use the same plasmid as tuesday but cut it with EcoRI to see if this restriction site worked.
3. We wanted to try to cut a new plasmid (RFP) with the same enzyme for the same amount of time (5 minutes), to find out if the enzyme worked or not.

Thursday 19/6

To be written.

Friday 20/6

Team page and Notebook page added to the wiki.