Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote
CODON OPTIMIZATION FOR HRPS AND HRPR GENE
Figure1: Codon Adaptation of hrpS gene in E.coli before optimization
CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%
Figure2: Codon Adaptation of hrpS gene in E.coli after optimization
CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%
Figure3: Codon Adaptation of hrpR gene in E.coli before optimization
CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70
Figure4: Codon Adaptation of hrpR gene in E.coli after optimization
CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%
reference
[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33
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