Team:INSA-Lyon/notebook

• 

• PROJECT

  • 
    PROJECT
  • PROJECT SUMMARY
  • ACHIEVEMENTS

• WETLAB

  • 
    WETLAB
  • WETLAB SUMMARY
  • RESULTS
  • NOTEBOOK
  • PROTOCOLS
  • DATA PAGE

• MODELING

  • 
    MODELING
  • MODELING SUMMARY
  • MOLECULAR MODELISATION
  • CURLI SYNTHESIS
•   HUMAN PRACTICE
  • 
    HUMAN
    PRACTICE
  • HUMAN PRACTICE SUMMARY
  • SYNBIO PERCEPTION
  • PROJECT'S INTEREST

• TEAM

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    TEAM
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  • HAVE FUN WITH IGEM
  • GALLERY

NOTEBOOK

February-June

Literature searches were performed about Peptide Display, Curli biogenesis and metal trapping.

June

11/06

Reception of the four DNA constructions: CsgA Wild type, CsgA PolyHis1, CsgA PolyHis2, CsgA Modular

24/06

NM522 and DH5α cells (in liquid LB medium) were cultured overnight under agitation at 37°C for later transformation.

25/06

25/06

25/06

Transformation of NM522 and DH5α strains with each one of the following plasmids.

Selection of transformed bacteria on antibiotic plates depending on the plasmid.

26/06

Transformation analysis: all the transformations were successful except the ones with plasmids pHC03, pHC06, pHC07 and pHC09. Transformation of these four plasmids was repeated with a new protocol.

Clones of transformed strains containing pHC01, 02, 04, 05 and 08 were cultured in 5 mL LB medium containing 50 µL of the specific antibiotic at 37°C and appropriate plates were streaked with isolated colonies likely to contain transformed bacteria.

27/06

Transformation analysis: all the transformations were successful and plates containing pHC06 plasmids showed pink bacteria, as the rfp in the plasmid is expressed in presence of a strong promoter (like J23100).

A protocol for nickel titration was searched and nickel was stored for later experiments.

25/06