Inoculate 10 mL of LB with frozen stocks of the three transformed clones (I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 electrocompetent cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm.
The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm.
Add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate.
Measurement Protocol
The samples were plated on a 96-well plate as follows:
The 96-well plate was inserted into the Infinite 200 PRO Microplate Reader
Open Tecan i-control, the software used to document the readings
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