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From 2014.igem.org
Revision as of 01:08, 9 October 2014 by
Andychau2015
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Home
UW 2014
UW 2013
UW 2012
UW 2011
UW 2010
UW 2009
UW 2008
iGEM Homepage
Our Project
Our System
Methods
Results
Future Plans
BioBricks
Outreach
Protocols
Safety
Safety
Project Considerations
Judging Form
Our Team
Background
Our System
Method
Analysis of Test Protein Stability
Cloning in
Escherichia coli
Preparation and Passaging of
Saccharomyces cerevisiae
Relative Stability Analyzed via Flow Cytometry
Mutagenesis through Error Prone Polymerase Chain Reactions
Selecting Stable Variants through Fluorescence Activated Cell Sorting
Results
Protein stability analysis using Thermal and Guanidinium Hydrogen Chloride melts
Protein stability analysis using Degron Constructs and Flow Cytometry
Mutagenesis Results and Mutant Variant Analysis
Future Plans
Examination of more proteins
Submitted Parts
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