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From 2014.igem.org

Contents 1 Protocols 1.1 Media, Plates and Solutions 1.1.1 CCMB 1.1.2 LB-Agar 1.1.3 Luria Broth 1.1.4 Super Optimal Broth (S.O.B.) 1.2 Basic Cloning 1.2.1 Polymerase Chain Reactions 1.2.2 Restriction Endonuclease Reaction (Digestion) 1.2.3 Ligation 1.3 Escherichia coli Protocols 1.3.1 Competent Cell Culturing 1.3.2 Transformations 1.3.3 Plating 1.3.4 Overnights 1.3.5 DNA-Extraction and mini-preps 1.4 Saccharomyces cerevisiae 1.4.1 Transformations 1.5 Flow Cytometry and Fluorescence Activated Cell Sorting 1.6 Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts

Protocols

Media, Plates and Solutions

CCMB

LB-Agar

Luria Broth

Super Optimal Broth (S.O.B.)

Basic Cloning

Polymerase Chain Reactions

Restriction Endonuclease Reaction (Digestion)

Ligation

Escherichia coli Protocols

Competent Cell Culturing

Transformations

Plating

Overnights

DNA-Extraction and mini-preps

Saccharomyces cerevisiae

Transformations

Flow Cytometry and Fluorescence Activated Cell Sorting

Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts

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