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Team:Kyoto
From 2014.igem.org
Project Overview
Clouds, which gives us rain and shade is the very important thing on the Earth. Some scientists say that coral and some other sea bacteria produce DMS, dimethyl sulfide which turns to be a seed of cloud. We focused on this interesting phenomenon. What we do is to create the E.coli which can produce DMS. In order to do so, we introduce the biosynthetic pathway of DMS into E.coli and detect the products by HPLC and DMS detector tube. Don’t you think it is fantastic if the small creature, E. coli, creates the big things, clouds? And this projects may have the possibility to answer the environmental problem.
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Project Overview
The purpose of our project is make magnetotactic bacteria’s organelle “magnetosome” in E. coli. Magnetotactic bacteria make magnetite in magnetosome. If E. coli have this bio mineralization system, we are able to move E. coli freely by magnetic force. It is known that many proteins are related to magnetosome formation but specific role of all magnetosome protein is not clear so we transform three proteins’ genes mamL, mamQ and mamB that are considered to be related first step of magnetosome formation. Finely we succeed to confirm lipid bilayer like magnetosome by TEM.
