Project Description

Fast and Accurate Biological Chromatic Sensing-Imaging System(FABCSIS)

Destination

Our project, Fast and Accurate Biological Chromatic Sensing-Imaging System(FABCSIS), was triggered by careful investigation of bacteria imaging study and light inducible responsing both in recent research and iGEM team work. However, we haven't found any teams focusing on using light sensoring-responing system to realize colorful pictures right in the media. Consequently, we decided to make it come true.

Coincidently, the red, blue, and green(RBG) light sensing proteins as well as RBG fluorescent proteins have been found in the latest study. As a result, what we are going to do are to integrate parts firmly by respectively guiding the three primary color light sensing-responsing system into engineering bacteria to achieve the photoreception and expression of corresponding color florescent proteins and then utilizing customized projector to view picture colorfully in the media and to improve the accuracy and speed of whole system by using Caulobacter cresentus as pedestal and ribozyme regulation.

More Accurate

To make the imaging system more precise, we attempt to use Caulobacter Crescentus, a kind of bacterium which has two types, stalked and swarmer cell, in whole lifestyle mainly for nutrition supplies. The stalked type, which has an adhesive holdfast, a complex polysaccharide composed in part of N-acetylglucosamine, can localize and stick to film tightly and irreversibly.

Using this special life cycle, we will let diverse bacteria percept disparate light in sessile phase and response with expression of RBG florescent proteins in stalked stage, which advance the accurate operation of the whole imaging system in many kinds of surroundings especially in the water flow.

Faster

We are trying to enhance the light responsing speed by remounding the gene regulation circuits with the use of RNA as many natural or engineering ribozymes have been found or constructed nowadays and the principal advantages of RNA regulation is to omit the translation process and regulate the gene expression level highly specificly. Therefore, designed ribozymes will be utilized into cell for swifter gene expression.

To View Colorfully

The FABCSIS,if oprating as we wish, will realize the chromatic imaging system firstly held in the media, regarding each individual as a pixel just like camera, by regulating life cycle of holdfast-possessing C.crescentus and appropriate ribozymes regulation. We believe our work will drive the further study and development of imaging system in other living organisms and one day biological cameras will appear in everybody's daily life to change the world for its lowcost and high resolution.