Team:Goettingen/proLP

Overview

PCR Methods

• Phusion PCR
• Gradient PCR

DNA Assessment

• DNA Gel Electrophoresis
• Pretreatment of DNA for Sequencing
• Fluorescence Assay

DNA Purification

• Gel Extraction

Plasmid Construction

• LB recombination reaction
• RB recombination reaction

Plasmid Transformation

• Preparation of competence cells
• Heat-shock transformation

Colony Scanning

• Yeast-Two-Hybrid System