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From 2014.igem.org

Contents 1 Lab Work 1.1 D-Lemon scent 1.1.1 Extraction of plasmid (liquide culture of Yesterday) 1.1.2 SalI digestion 1.1.3 ligation

Lab Work

by Mélanie

D-Lemon scent

Extraction of plasmid (liquide culture of Yesterday)

and PacI digestion to check another time if we really obtained our plasmid

AND YES it is here ! We had obtained pPS3 and pPS4 !

SalI digestion

In order to obtain pPS5, I digestion by SalI the pPS4 plasmid and the topo-CAD plasmid

ligation

ligation of pPS4 and CAD

(transformation will occure on monday)