Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep

Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis

Date:8/25 PCR and Electrophoresis and Restriction

Date:8/26 Insert gene into TAvectar and Transformation

Date:8/26 Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction

Creating parts of STAT3

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis

Date:8/20 PCR(Lowered 2℃ from Tm value.) and Electrophoresis

Date:8/21 PCR and Electrophoresis

Date:8/22 PCR and Electrophoresis

Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)

Date:8/26 PCR and Electrophoresis(Using a mutation primer.)

Date:8/27 First Variation introduction (with Prime STAR Max)

Date:9/1

Date:9/2

Date:9/8 PCR and Electrophoresis

Date:9/9 Sequence(to To confirm whether variation happened in DNA of STAT3 )

Date:9/10~9/12 PCR and Electrophoresis

Creating parts of IL-10α,IL-10β

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis

Date:8/20 PCR(Lowered 2℃ from Tm value.) and Electrophoresis

Date:8/21 PCR and Electrophoresis

Date:8/22 PCR and Electrophoresis

Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)

Date:8/26 PCR and Electrophoresis(Using a mutation primer.)

Date:8/27 First Variation introduction (with Prime STAR Max)

Date:9/1

Date:9/2

Date:9/8

Date:9/9 Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β)

Date:9/10~9/12 PCR and Electrophoresis