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Team:UCL/Lab AzoReducateCloning Portal
From 2014.igem.org
BioBrick and Primer Design
Two Azoreductase enzymes will be cloned from Bacilus subtilis (strain 168) and Pseudomonas Aeruginosa (PA01) using DNA PCR amplification.
Suitable forward and reverse primers were designed for each enzymes using the following strategy.
The protein coding sequences for each enzyme were obtained from ?.
iGM SUBF4 : 5’-ATGTCTACAGTTTTATTTG-3’ iGM PRESUB4 : 5’-GAATTCGCGGCCGCTTCTAGATGTCTACAGTTTTATTTG-3’ iGM REVSUB:5’-TTAGAATTTTGCAGCAAGA-3’ iGM SUF REV SUB:5’-TACTAGTAGCGGCCGCTGCAGTTAGAATTTTGCAGCAAGA-3’
iGM AER4 : 5’ATGAGTAGAATTCTTGC-3’ iGM PRE AER4: 5’-GAATTCGCGGCCGCTTCTAGATGAGTAGAATTCTTGC-3’
iGM REV AER :5’-TCAGGCCGACCGCGCCAG-3’ iGM SUF REV AER: 5’-TACTAGTAGCGGCCGCTGCAGTCAGGCCGACCGCGCCAG-3’
