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From 2014.igem.org

Week 14

Monday, August 25th

• We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.

• We have a Comsol simulation for this experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.

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