Team:Cambridge-JIC/Enhancer Trap

Revision as of 15:12, 22 August 2014 by Wtk23 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

• Welcome
  • Home
  • Project Overview
  • Meet the Team
  • Official Team Profile
• Introducing Marchantia
  • Why Marchantia?
  • Background on Bryophytes
• Labwork
  • Notebook
  • Chromoproteins
  • Raspberry Ketones
  • Hammerhead Ribozyme
  • Enhancer Trap
  • Protocols
  • Dry Work
• Bioinformatics
  • Codon Optimisation
  • Promoter Screen
• Technology
• Parts and Results
  • Parts
  • Results
• Outside the lab
  • Preparing for iGEM
  • Getting the word out
  • Outreach
  • Socials
• Safety and Considerations
• Attributions
  • Acknowledgements
  • Sponsors
• iGEM Home
  • iGEM Home

Enhancer Trap

Prologue

As a relatively new chassis for synthetic biology, Marchantia polymorpha has few promoters and enhancers. The viral promoter '35S' and the '?' are the only highly tested promoters for each. Consequently, we are randomly integrating a plasmid with a minimal promoter driving a GFP gene into the Marchantia polymorpha genome, and observing expression levels. After screening several thousand transformants for sex-specific/developmental-specific/highly strong promoters, we will reverse PCR several kb upstream from the inserted genes. These shall then be released as parts, if successful.

Lab Notebook

Objective: randomly integrate a plasmid with a minimal promoter into the Marchantia genome, using a Venus flo-protein reporter. Then screen, and establish enhancer lines which are tissue or developmental stage specific, or of great strength.