Team:Paris Bettencourt/Notebook/Foot Odor

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Paris Bettencourt 2014



Foot Odor

24-06-2014 - Re-Streaking wt. B.subtilis on LBA to obtain single colonies

AIM: To obtain single colonies of Wt b.Subtilis from 23-06-14 plate

we took a single colony and streaked it on a new LBA with and w/o Erythromycin.

Observation:

No colonies in LBA + EM
Colonies observed in LBA w/o EM

 

 

27-06-2014 - Preparing LBA and LB broth with Erythromycin

AIM: To prepare LBA and LB broth for B.Subtilis mutants

We added 2.5 ul of the EM (20mg/ml) to 50ml of LB broth. We added 25 ul of EM to 250 ml of LBA and prepared the plates & stored them at 4° C.

 

 

30-06-14 - Preparation of miniculture of wild type and mutant B.Subtilis for glycerol stock

AIM: To prepare 10% glycerol stock of wt and mutant (BKE24040, BKE24050, BKE24080) B.Subtilis

We inoculated  Single colony of both wt (1A1) and mutant (BKE24040, BKE24050, BKE24080) in 5ml of LB broth supplemented w/o EM for wt and with EM for mutants. Incubated the colonies @ 37°C overnight.

After the overnight incubation the tubes were stirred well. 750 ul of the culture is transferred to cryogenic tube and 750 ul of 60% glycerol is added to the culture to obtain 10% glycerol stock.

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June

23-06-2014 1
Goal: To obtain single colonies of both Wt and mutant B.Subtilis

We received the wt (1A1) and mutant BKE24040, BKE24050, BKE24080 from BGSC on '''23-06-2014'''. The wt strain (1A1) was on a paper strip. We received 3 strips from BGSC (Bacillus genetic stock centre).

Procedure

We plated two strips in two different LBA plates. The plates were free from antibiotics. We placed the strips gently on the LBA and added 1 drop of sterile H2O. After few seconds we streaked the paper strip on the LBA and discarded the strip. The LBA plates were incubated at 37° C.

Results

Good growth of the wt B.subtilis was observed followed by the overnight incubation

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