Team:Evry/Notebook/Sensing/PCBs/week5
From 2014.igem.org
Week 5
Sensor construction bphR1/bphR2
07/27/14:
bphR1 promoter:- Culturing bphR1 promoter BBa_K1155001, received from Saclay's team, in 5ml LB + 5µL Chloramphenicol (Cam)
- Incubation at 37°C overnight
07/28/14:
bphR1 promoter:- Plate of 1 colony on LB-agar-Cam (25 mL LB-Agar + 25µL Cam)
- Incubation at 37°C overnight and glycerol stock