Team:Lethbridge
From 2014.igem.org
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Project Summary | |||||||||
Every year, traumatic brain injury, stroke and neurodegenerative disease collectively cost the Canadian medical system millions of dollars, and effective neural regenerative therapies remain elusive. Astrogliosis, a cellular response common to such neural insults, leads to the formation of a non-functional “glial scar” and an inhibitory cellular environment that impedes neural regeneration and further recovery. | |||||||||
Our proposed project involves engineering microglia, the mobile immune cells of the brain, to package and deliver a therapeutic plasmid DNA construct specifically to the reactive astrocytes that comprise these glial scars. The plasmids will contain a reprogramming factor that converts reactive astrocytes into new neurons, helping to both restore the functional neuronal population and permit regrowth of damaged connections. | |||||||||
Our specific objectives include:
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Altogether, this study provides a novel, tissue-specific, personalized, non-immunogenic, non-invasive neural rehabilitative therapy that has the potential to significantly improve current methods of stimulating functional recovery following brain injury or disease onset. The long-term objective of this proposal is to significantly improve current neural regeneration therapies in an effort to overcome the rehabilitative obstacles associated with CNS insults such as stroke, Alzheimer’s Disease, and traumatic brain injury in a non-invasive, cost-effective manner. Furthermore, with future discoveries of other novel tissue-specific tags coupled with targeted DNA transmission therapies such as that discussed in this study, this system can potentially be harnessed to combat other tissue-specific disorders. | |||||||||