In the second module we aim to use a carboxysome which is found in cyanobacteria or purple sulfurbacteria. With this compartment we want to create a Calvin-Benson cycle in E.coli. In addition we would like to compare the efficiency of the carboxysome with a free RuBisCO (Ribulose-1,5-bisphosphate-carboxylase-oxygenase), the 3-Hydroxypropionate cycle or other types of carboxysomes. The product of the fixation will be pyruvate which can be used for production of different metabolites like for example Isobutanol.

Here you will find the results of the CO₂ fixation.