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From 2014.igem.org

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Contents 1 The different parts of our project: 1.1 A - The chassis coli Odor free 1.2 B - Construction of the fusion protein (color) 1.3 C - Salicylate Inducible Suppressing System 1.4 D - Lemon scent 1.5 E - Banana scent 1.6 F - The lemon shaping

The different parts of our project:

A - The chassis coli Odor free

Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene.

In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but this strain was too modified to be used for our project. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.

B - Construction of the fusion protein (color)

C - Salicylate Inducible Suppressing System

D - Lemon scent

• PCR Cleanup of BBa_K762100 and BBa_K517003 - 6th August

E - Banana scent

F - The lemon shaping